Elucidation of the potential antioxidant compound and mechanism of mung bean using network pharmacology and in vitro anti-oxidative activity

Mung bean is rich in bioactive components, but the main compound and pharmacological mechanism reducing oxidative free radical damage are unclear. Network pharmacology 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) scavenging activities were employed to uncover antioxidant of potentially active compounds, considering interactions between mung targets damage. These key analyzed by protein–protein (PPIs), genes used find biological pathway therapeutic Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analyses. The results showed that five components 18 screened. β-carotene vitexin both played a crucial role against damage, ABTS 94.84 87.79%, which equivalent those vitamin C. Key may be AR, HSP90AA1, MYC, CASP3 for exert activity. GO KEGG indicated mainly act on thyroid hormone signaling pathway, estrogen p53 etc. In vitro activity tests ingredients beans had great analysis also revealed underlying molecular mechanisms This study provides new insights evidence explore compounds functions food cereals legumes, as well reference functional evaluation development foods.